Canine Heart Health, and Longevity

Healthy dogs do not typically experience issues related to fluid retention or protein accumulation [1]. However, as dogs get older, the heart’s ability to sufficiently pump blood throughout the body typically decreases. This can lead to fluid retention as well as the reduced circulation of oxygen- and nutrient-rich blood to vital areas of the body including the intestines [2]. When this occurs, the digestive tract may struggle to break down proteins into nutritional amino acid building blocks, thereby allowing large portions of protein to be excreted through the stool [3]. Furthermore, as the dog’s body tries to address the fluid retention and digestive issues, the heart will be placed under additional stress. As a result, your dog can have reduced energy and cardiovascular health and may be losing vital nutrients.

Canine Bloating

Fortunately, research shows that dietary supplementation with enzymes can support healthy heart and digestive function[4, 5]. Neprofin is a powerful blend of enzymes that offer a natural approach to supporting normal circulation and the digestion of circulating proteins. This potent, all-natural formula was designed specifically for dogs to maintain healthy fluid levels, blood flow, protein metabolism, and proper waste removal. It contains powerful, protein-dissolving enzymes such as bromelain and serrapeptase.

A number of studies have shown that bromelain supplementation disrupts fluid retention along with the discomfort that accompanies it by supporting the normal transfer of nutrients from the intestines to the bloodstream [4-6]. More specifically, bromelain breaks proteins down into smaller amino acids that can easily travel through the digestive tract and into the blood, thereby allowing them to be reabsorbed as nutrients. This process also maintains normal intestinal fluid thickness, which subsequently reinforces normal digestion and the natural movement of fluid away from the abdomen and into the blood circulation [6].

Serrapeptase supports normal fluid transfer in a similar manner to bromelain, and it also has properties that lessen discomfort from occasional fluid retention [7, 8]. Several of the other enzymes and antioxidants in Neprofin such as amla, papain, rutin, and protease have similar and powerful activity as well [9-16]. In addition to fortifying digestion and fluid movement, Neprofin supports heart function by supplying a dog’s body with enzymes that support normal and healthy cholesterol and triglyceride levels. One such enzyme is lipase, which breaks down fat in all areas of the body, including the arteries [17, 18]. While lipase is naturally produced by the body, many lifestyle, genetic, and health factors can drastically reduce its production. Fortunately, oral lipase supplementation helps maintain optimal levels of this beneficial enzyme.

Fibrin in Dogs

Fibrin Defense

Fibrin is a major component of scar tissue and blood clotting. Younger healthy pets maintain a normal fibrin equilibrium, which maintains clean, healthy blood. However, aging mammals produce less of the necessary blood regulating enzymes over time making it more difficult to maintain proper blood health.

Neprofin contains a blend of fibrinolytic enzymes that support healthy blood viscosity. When healthy, canines normally secrete sufficient levels of plasmin, an enzyme that acts as a natural blood cleanser. Plasmin is responsible for maintaining normal blood viscosity by removing unnecessary proteins, specifically fibrin. Neprofin—like many fibrinolytic enzymes—supports healthy fibrin levels.

Canine Longevity and Heart Health

Overall, Neprofin enhances a dog’s quality of life by supporting normal fluid retention, blood flow, and lessening discomfort due to occasional swelling. The specifically chosen enzymes reinforce digestion and heart health, which is especially important for older dogs. Therefore, regular supplementation with Neprofin can help give your dog years of happiness and longevity.



  1. O’Brien PJ, Lumsden JH. The cytologic examination of body cavity fluids. Semin Vet Med Surg. 1988;3:140-156.
  2. Odze RD, Goldblum JR. Surgical pathology of the GI tract, liver, biliary tract, and pancreas. 2nd ed. Philadelphia, PA: Saunders Elsevier; 2009: 181.
  3. Umar SB, DiBaise JK. Protein-losing enteropathy: case illustrations and clinical review. Am J Gastroenterol. 2010;105:43-49.
  4. Maurer HR. Bromelain: biochemistry, pharmacology and medical use. Cell Mol Life Sci. 2001;58:1234-1245.
  5. Netti C, Bandi GL, Pecile A. Antiinflammatory action of proteolytic enzymes administered orally compared with antiphlogistic compounds. Il Pharmaco. 1972;27:453-466.
  6. Smyth RD, Brennan R, Martin GJ. Systemic biochemical changes following the oral administration of a proteolytic enzyme, bromelain. Arch Int Pharmacodyn. 1962;136:230-236.
  7. Tachibana M, Mizukoshi O, Harada Y, et al. A multi-centre, double-blind study of serrapeptase versus placebo in post-antrotomy buccal swelling. Pharmatherapeutica. 1984;3:526-530.
  8. Mazzone A, Catalani M, Costanzo M, et al. Evaluation of Serratia peptidase in acute or chronic inflammation of otorhinolaryngology pathology: a multicentre, double-blind, randomized trial versus placebo. J Int Med Res. 1990;18:379-388.
  9. Asmawi MZ, Kankaanranta H, Moilanen E, Vapaatalo H. Anti-inflammatory activities of Emblica officinalis Gaertn leaf extracts. J Pharm Pharmacol. 1993;45:581-584.
  10. Ihantola-Vormisto A, Summanen J, Kankaanranta H, et al. Anti-inflammatory activity of extracts from leaves of Phyllanthus emblica. Planta Med. 1997;63:518-524.
  11. Lambertini E, Lampronti I, Penolazzi L, et al. Expression of estrogen receptor alpha gene in breast cancer cells treated with transcription factor decoy is modulated by Bangladeshi natural plant extracts. Oncol Res. 2005;15(2):69-79.
  12. Leipner J, Iten F, Saller R. Therapy with proteolytic enzymes in rheumatic disorders. BioDrugs. 2001;15(12):779-789.
  13. Leipner J, Saller R. Systemic enzyme therapy in oncology: effect and mode of action. Drugs. 2000;59(4):769-780.
  14. Hale LP, Greer PK, Sempowski GD. Bromelain treatment alters leukocyte expression of cell surface molecules involved in cellular adhesion and activation. Clin Immunol. 2002;104:183-190.
  15. Selezneva AA, Bol’shakova MD. Proteolytic complex from Aspergillus terricola. Prikl Biokhim Mikrobiol. 1986;22(1):3-11.
  16. Selezneva AA, Babenko GA, Bol’shakova MD, Rozhanskaia TI, Margolina NA. Preparative isolation of terrilytin components and study of their enzymatic properties. Prikl Biokhim Mikrobiol. 1976;12(3):416-420.
  17. Du H, Schiavi S, Levine M, Mishra J, Heur M, Grabowski GA. Enzyme therapy for lysosomal acid lipase deficiency in the mouse. Hum Mol Genet. 2001;10(16):1639-1648.
  18. Hall DA, Zajac AR, Cox R, Spanswick J. The effect of enzyme therapy on plasma lipid levels in the elderly. Atherosclerosis. 1982;43(2):209-215.
  19. American Heart Association, Official Report, Mat 1972.  Chen JR. In vivo and in vitro studies of the effect of bromelain on cholesterol-protein binding. Dissert. Abstr B. 1975;35(2 Pt) 6013, Ord. No. 75-13, 735.