Sleepbiotic™

CP2305 is a heat-inactivated Lactobacillus gasseri postbiotic studied in multiple human clinical trials for stress-related symptoms, sleep quality, and gut-brain axis support. Unlike a conventional probiotic, CP2305 does not depend on live bacteria colonizing the digestive tract. Because it is heat-inactivated, its microbial components remain stable and can interact with the gastrointestinal environment as a postbiotic.

In one randomized, double-blind, placebo-controlled study, 60 healthy medical students under chronic academic stress took CP2305 daily for 24 weeks. Compared with placebo, CP2305 significantly reduced anxiety (measured by the Spielberger State-Trait Anxiety Inventory) and improved sleep quality (measured by the Pittsburgh Sleep Quality Index). Sleep EEG recordings also showed a shorter time to fall asleep, less time awake after sleep onset, and a greater proportion of deep, slow-wave sleep in the first sleep cycle. Separate CP2305 research in adults under academic and occupational stress has also shown reductions in salivary cortisol and a shift toward a calmer nervous system state.

Melatonin is one of the most widely used sleep aids in the world, but most commercial melatonin is synthetic and manufactured through a multi-step chemical synthesis process rather than sourced from nature. Sleepbiotic instead uses melatonin extracted directly from tomatoes, one of many plants where melatonin occurs naturally.

Melatonin is a hormone that helps synchronize the body’s circadian rhythm and cue the onset of sleep. Rather than using a higher dose, Sleepbiotic contains a deliberately low 1 mg dose. Research suggests this level can support sleep onset without pushing melatonin to blood levels that can linger into the morning and contribute to next-day grogginess.

Glycine is the smallest and simplest amino acid in the body, but it plays an outsized role. It’s a building block for collagen and other proteins, and it acts as an inhibitory neurotransmitter in the brain and spinal cord. For sleep, glycine has a distinct mechanism: it acts on NMDA receptors in the suprachiasmatic nucleus (the brain’s master clock) to gently lower core body temperature, one of the body’s natural signals that it’s time to sleep. In published research, glycine taken before bed has been shown to help people fall asleep faster, spend more time in deep, restorative sleep, and feel less fatigued the following day without altering the body’s normal sleep architecture the way many sedatives do.

L-theanine is a water-soluble amino acid found abundantly in green tea leaves. Once it reaches the central nervous system, research suggests it influences several neurotransmitter systems at once, helping raise GABA and glycine activity while modestly supporting dopamine and serotonin turnover. The combined effect is calm, clear-headed relaxation. L-theanine helps ease a racing mind and reduce stress without the sedation or next-day fog associated with many calming ingredients.

GABA (gamma-aminobutyric acid) is the brain’s primary inhibitory neurotransmitter. Its role is to calm neuronal activity, essentially acting as the nervous system’s brake pedal. In human trials, taking GABA orally before bed has been shown to shorten the time it takes to fall asleep and increase time spent in non-REM sleep, alongside measurable shifts toward calmer, more relaxed brainwave activity within about an hour of intake. Sleepbiotic includes GABA alongside L-theanine and glycine to round out its calming, stress-easing effect.

Sleepbiotic is a combination of five active ingredients formulated to help you relax and drift into a restful night’s sleep. The formula is anchored by the postbiotic CP2305 and a low dose of plant-derived melatonin, both shown to support restful sleep.

Sleepbiotic is intentionally formulated to minimize next-day grogginess. Its melatonin dose is low but still clinically effective and hasn’t been associated with morning grogginess in research. The rest of the formula focuses on easing stress and supporting relaxation rather than sedation.

One could argue that synthetic melatonin is chemically the same as the melatonin naturally produced by the body, and that where it comes from or how it is made is irrelevant. But when you look more closely at how synthetic melatonin is produced, you uncover a chemical synthesis process that can involve some or all of the following, and this list is not exhaustive: 1,3-dibromopropane, acetone, potassium carbonate, phthalimide, sodium ethoxide, ethyl acetoacetate, 2-carboxyethyl-3-(2-phthalimidoethyl)-5-methoxyindole (8), sodium hydroxide, and methanol.

“Better” may not necessarily be the word that best describes the difference between synthetic and plant-derived melatonin. But “cleaner” is one way to describe the difference when you consider their respective manufacturing processes and origins. In addition, the plant-derived melatonin found in Sleepbiotic also includes plant compounds such as lycopene and other phytonutrients.

Sleepbiotic works through five complementary ingredients rather than a single sedating compound. CP2305, a well-studied postbiotic, works through the gut-brain axis to support a healthier stress response and sleep patterns over time. A low 1 mg dose of plant-derived melatonin helps cue your body that it’s time to sleep. L-theanine, glycine, and GABA round out the formula, working together to quiet a racing mind, ease physical tension, and support restorative sleep. Together, these ingredients are designed to help you fall asleep, stay asleep, and wake up feeling rested while minimizing the next-day grogginess often associated with higher melatonin doses.

The formula does not contain any animal-derived components.

Yes. Sleepbiotic is non-GMO. None of the ingredients used to make this product are genetically modified organisms as defined by the National Organic Standards Board.

Genetically engineered is defined as follows: made with techniques that alter the molecular or cellular biology of an organism by means that are not possible under natural conditions or processes. Genetic engineering includes recombinant DNA, cell fusion, micro- and macro-encapsulation, gene deletion and doubling, introducing a foreign gene, and changing the position of genes. It shall not include breeding, conjugation, fermentation, hybridization, in-vitro fertilization, and tissue culture.